Cell cycle: regulatory mechanisms and biochemical markers
DOI:
https://doi.org/10.32635/2176-9745.RBC.1994v40n3.2983Keywords:
Cellular Cycle, Proliferative Cell, Cell-Cycle Markers, Proto-OncogenesAbstract
The regulatory mechanisms of the cellular cycle and its relationship with the growth factors, oncogenes end anti-oncogenes are one of the major advanced fields in the cancerbiology studies. The Identification of proteins and cofactors regulating the transitions between G1/S and G1/M as well the transcription of the genetic material have allowed the detection of cyclíng cells even before the appearance of the mitotic signs. The development of techniques using monoclonal antibodies against those proteins have made possible studies on proliferative cells of different tomours, mainly based on fact that proteins such as alpha polymerase and cyclin/PCNA are expressed only by proliferating cells. Thus the detection and counting of these cells would allow the evaluation of either the tumor-growth rate or its therapeutic efficiency and consequentiy the prognostic of the disease. The present paper reviewed the current knowledgement of the cell-cycle control mechanisms and the available methodology for the proliferative-cell detection emphasizing the biochemical markers, its practicability and reliability.
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