Genotyping of Human Papillomavirus in patient with recurrent laryngeal papilomatose

Abstract

HPV is a circular double-stand DNA virus with approximately 8000bp. Some studies have implicated HPV as the etiological agent of benign tumors such as papilomas, commons warts, and condilomas. With the advance of molecular techniques, detection of the HPV genome has been made fairly easy and has been frequently identified in malign neoplasic cells of epithelial origin, associating the HPV with some epithelials tumors, mainly with cervical carcinoma. Recently, several studies have been aiming to understand the role HPV infection in the initiation and promotion of head and neck cancers. The following article consists of a case report of a patient with history of respiratory recurrent papillomatosis. A 7-year old female patient was examined upon complaint of discomfort and pain in her throat, including disphonic episodes. Common clinical procedures indicated the presence of papilomas in her larynx. The histopathological analysis of the papilomas revealed coilocytosis, suggesting HPV infection. The patient underwent a polypectomy and the tissue was used to extract total DNA for the molecular investigation of the HPV genome by PCR. The results confirmed the presence of HPV11, corroborating findings reported elsewhere that implicated HPV11 as a participant in the evolution of benign recurrent respiratory papillomatosis in children.