Detecção Imuno-Histoquimica de Antígenos de Superfície ABH em Carcinoma de Células Transicionais da Bexiga: Descrição do método e comparação com a histologia clássica
DOI:
https://doi.org/10.32635/2176-9745.RBC.1986v32n1.3228Keywords:
Bladder Carcinoma, Imunohistochemistry, ABH Surface AntigensAbstract
Deletion of ABH surface antigens is associated with malignant cell transformation in a variety of human neoplasms, including bladder carcinomas. Data from studies using the red cell adherence test (SRCA) suggested that iossof ABH antigens was associated with histologically high graded tumors, with a worse clinical outcome. However, the impact of these findings was attenuated due to the drawbacks of the technique. Based on the extraordinary accuracy of immunohistochemistry, the present study was performed using a Ulex europaeus lectin to detect ABH antigens, with ampiification mediated by the avidin-biotin-peroxidase complex. The main goals were the evaluation of the applicability of this method and the correlation of the presence of antigens with histoiogicai grading. Thirty-nine (59,1%) out of 66 specimens evaluated were positive for ABH antigens. 85% of grade I tumors were positive, and "strong" staining was achieved in 69,2%. Grade II tumors showed 82,7%, of positivity and this was “strong" only in 58,6%. On the other hand, only 17,0% high-graded lesions (grade III) were positive and, in this group "strong" staining was obtained only in 12,5%. Our results showed that this new technique overcame the drawbacks of the red cell adherence test and its quite applicable to formalin-fixed paraffin embedded tissues. This technique, together with other prognostic parameters, can be very heipfui in the elaboration of therapeutic strategy of patients with transitional cell carcinoma of the bladder.
Downloads
References
Srougi M, Góes GM. — Câncer urológico. II — Câncer de bexiga. J Bras Urol 1984; 10:110-116.
Brumini R, Torloni H, Henson DE, Gotlieb SLD, Souza JMP. Câncer no Brasil. Dados histopatológicos 1976-1980. Ministério da Saúde, Rio de Janeiro, 1982; 231.
Silverberg E. — Cancer statisties, 1985. Ca-A Câncer J 1985; 35:19-35. DOI: https://doi.org/10.3322/canjclin.35.1.19
Hastings RJ, Franks LM. — Celular heterogeneity in a tissue culture cell live derived from a human bladder carcinoma. BR J Câncer, 1983; 47: 233-244. DOI: https://doi.org/10.1038/bjc.1983.31
Slack NH, Prout GR, JR. — The heterogeneity of invasive bladder carcinoma and different responses to treatment. J Urol 1980; 123:644-652. DOI: https://doi.org/10.1016/S0022-5347(17)56075-9
Coombs RRA, Belford D, Rouiliard LM. — A and B blood-group antigens on human epidermal cells, demonstrated by mixed agglutination. Lancet, 1956; 1 :461-463. DOI: https://doi.org/10.1016/S0140-6736(56)90528-1
Glynn LE, Holborow EJ. — Distribution of blood-group substances in human tissues. Br. Med BulI, 1959; 1 5:1 50-1 53.
Szulman AE. — The histological distribution of blood group substances A and B in man. J Exp Med 1960; 11:785-799. DOI: https://doi.org/10.1084/jem.111.6.785
Kay HEM. — A and B antigens of normal and malignant cells. Br J Cancer, 1957; 11:409-414. DOI: https://doi.org/10.1038/bjc.1957.49
Davidsohn I, Stejskal R, LilI P. — The loss of isoantigens A, B, and H in carcinoma of urinary bladder. Lab Invest, 1973; 28:382.
Kovarik S, Davidsohn I, Stejskal R. — ABO antigens in cancer. Detection with the mixed cell agglutination reaction. Arch Pathol, 1968; 86: 12-21.
Bishop MC. — Blood group antigens and bladder cancer. Br Med J, 1982; 284: 1426-1427. DOI: https://doi.org/10.1136/bmj.284.6327.1426-a
Gunter PA, Abela-Borg J, Pugh R.B.C. - Urothelium and specific red cell adherence test. Br J Urol, 1983; 55: 10-16. DOI: https://doi.org/10.1111/j.1464-410X.1983.tb07070.x
Lange PH, Limas C. — Tissue blood group antigen testing in transitional cell carcinoma of the bladder. J Urol, 1980; 124:304. DOI: https://doi.org/10.1016/S0022-5347(17)55421-X
Limas C, Lange P. — Altered reactivity for A, B, H antigens in transitional cell carcinomas of the urinary bladder. A study of the mechanisms involved. Cancer, 1980; 46: 1366-1373. DOI: https://doi.org/10.1002/1097-0142(19800915)46:6<1366::AID-CNCR2820460613>3.0.CO;2-#
Alves VAF, Moraes CF, Santos RTM. Takeda AK, Gayotto, L. C. C. Utilização da técnica de anticorpos não marcados (PAP) na detecção do antígeno de superfície da hepatite B em tecido hepático. Rev Inst Adolfo Lutz, 1980; 40 (2): 101-6.
Alves VAF, Boise W, Abbo AH, Mendonça BB, Bisi H. — Identificação da origem de metástases de carcinoma da tireóide através do método da imunoperoxidase. Arq Bras Endocrinol Metabol 1984; 28 (1):17-19.
Hsu SM, Raine LM, Fanger H. — The use of antiavidin antibody and avidin-biotin-peroxidade complex in immunoperoxidase technics. Am J Clin Pathol, 1981; 75: 816-821. DOI: https://doi.org/10.1093/ajcp/75.6.816
Kim YS, Isaacs R, Perdomo JM. — Alterations of membrana glycopeptides in human colonic adenocarcinoma (lectins/glycosyltransferases/glycosidades). Proc nat Acad Sci USA, 1974;71: 4869-4873. DOI: https://doi.org/10.1073/pnas.71.12.4869
Steele JG, Rowlatt C, Sandall JK, Franks LM. — Identification of exposed surface glycoproteins of human bladder carcinoma cell lines. Biochim Biophys Acta, 1983; 732: 219-228. DOI: https://doi.org/10.1016/0005-2736(83)90206-7
Bali JP, Magous R, Lecou C, Mousseron-Canet M. — Presence of blood group Fl on a carcinoembryonic antigen, and its enzymatic modification into blood group A and B specificities. Câncer Res, 1976; 36: 2124-2129.
Hakomori SI — Blood group ABH and lI antigens of human erythrocytes: chemistry, polymorphism, and their developmental change. Semin Hematol 1981; 18:39-62, 1981.
Bergman S, Javadpour N. — The cell surface antigen A, B or O (H) as an indicador of malignant potential in stage A bladder carcinoma: preliminary report. J Urol, 1978; 119: 49-51. DOI: https://doi.org/10.1016/S0022-5347(17)57378-4
Finan PJ, Anderson JR, Doyle PT. Lennox ES., Bleehen, NM. — the prediction of invasive potencial in superficial transitional cell carcinoma of the bladder. Br J Urol, 1982; 54; 720-725. DOI: https://doi.org/10.1111/j.1464-410X.1982.tb13633.x
Cooper HS, D'Elia FL. - Comparison between the methods of indirect immunofluorescence and specific rec cell adherence test in detecting ABH isoantigens in bladder carcinoma. Am. J. Clin. Pathol. 1982; 77:548-554. DOI: https://doi.org/10.1093/ajcp/77.5.548
Das G, Buxton NJC, Glashan RW. — Invasive potencial of superficial bladder cancer. A study of the relative merits of predictive parameters Br J Urol, 1985; 57:32-36. DOI: https://doi.org/10.1111/j.1464-410X.1985.tb08979.x
King CT, Clark TD, Lovett J, Cash JB, Primus FJ, McRoberts J, et al-A comparison of clinical course with blood group antigen testing by specific red cell adherence and immunoperoxidase in uretral and renal pelvic tumors. J Urol, 1983; 130: 871-873. DOI: https://doi.org/10.1016/S0022-5347(17)51543-8
Usu, SM, Raine L. — Versatily of biotin-labeled-lectins and avidin-biotin-peroxidase complex for localization of carbohydrate in tissue sections. J Histochem Cytochem, 1982; 30: 157-161. DOI: https://doi.org/10.1177/30.2.7037937
Hsu, SM, Raine L, Fanger H. — Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and uniabeled antibody (PAP) procedures. J Histochem, 1981; 29:577-580. DOI: https://doi.org/10.1177/29.4.6166661
Mac Alpine RG, Javadpour N, Vafier JA, Worsham GF, 0'Connel KJ. Immunoperoxidase versus specific red cell adherence in detection of ABO (H) antigens on normal urothelium. Urology 1984; 153-156. DOI: https://doi.org/10.1016/0090-4295(84)90417-5
AIroy J, Szoka FC, Heaney JA, Ucc, AA. — Lectins as a probe for carbohydrate residues in non-neoplastic urothelium of human urinary bladder. J Urol 1982; 128:189-193. DOI: https://doi.org/10.1016/S0022-5347(17)52815-3
Fenton JW, II, Duggleby CR, Otten C, Stone WH. — Isolation and fluorescent labeling of Ulex europaeus anti-H lectin. Vox Sang, 1965; 10:208-211. DOI: https://doi.org/10.1111/j.1423-0410.1965.tb04339.x
Lee AK, Rosen PP., DeLeIlis RA, Saigo PE, Groshen S, Bagin R., et al — Immunohistochemical tumor marker profile and survival in breast carcinoma. Am J Clin Pathol 1985; 84:687-696. DOI: https://doi.org/10.1093/ajcp/84.6.687
Lee AK, De Leilis RA, Wolfe HJ. — Intramammary lymphatic invasion in breast carcinomas — Evaluation using ABH isoantigens as endothelial markers. Am J Clin Pathol in press.
Koss LG. — Tumors of urinary bladder. In Atlas of tumor Pathology, Armed Forces Institute of Patology, Washington DC, 1975; 11S-46.
