Investigation of Single Strand Conformational Alterations of the TP53 Gene in Epithelial Hyperplasias of the Breast

Abstract

Generally, benign breast lesions behave as innocuous and limited proliferations, but sometimes they can represent pre-cancerous diseases. The practical importance of epithelial hyperplasias studies is related to their potential for malignant transformation. The TP53 tumor supressor gene suffers the greatest number of mutations in human cancer Using single strand conformational polymorphism, we did a mutation screening in exons 5 to 8 of the TP53 gene in the tumoral tissues of five patients with epithelial hyperplasias of the breast. The obtained results do not show any polymorphism that indicates mutation. The lack of mutation indicates that this gene is not involved in the intial process of malignization, strengthening the hypothesis that mutations on TP53 gene are a late event in the breast carcinogenesis.