Dosagem Colorimétrica da Lactodesidrogenase
DOI:
https://doi.org/10.32635/2176-9745.RBC.1960v17n22.3722Keywords:
L-Lactate Dehydrogenase/administration & dosage, NAD/administração & dosagem, Indicators and ReagentsAbstract
The author recommend a coloritric method for measuring the lactic dehydrogenase (LDH) activity in 0,1 ml of serum based on the reation lactate + DPN à pyruvate +\ DPNH2 at pH 10 glicina buffer. The pyruvate formed meseared by dinitro-phenil-hidrazine in alcaline médium. As standart color they use pyruvate solution or artificial standart - Natrium salicilate + Iron cloride solution.
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References
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