Comparação dos parâmetros cinéticos da xantina desidrogenase em neoplasia experimental
DOI:
https://doi.org/10.32635/2176-9745.RBC.1990v36n1/4.3125Palabras clave:
Xantina, Desidrogenase, Sarcoma, 20-MetllcolanrrenoResumen
O objetivo do presente trabalho foi o de comparar os parâmetros cinéticos da xantina desidrogenase (XD) do soro de ratos normais e de ratos protadores de sarcoma induzido por 20-metilcolantreno. Foi verificado que a XD decresce no soro dos animais portadores. A afinidade da XD para com a xantina foi semelhante no soro normal e no soro de animais com sarcoma. A inativação a 7O C é, aparentemente, de primeira ordem em relação à concentração de enzima. A atividade XD foi linear durante 60 minutos de incubação e a enzima do soro de animais com sarcoma foi mais sensível à ação de diferentes inibidores. Concluiu-se não haver diferença significante na estrutura de ambas as XD e que o decréscimo da atividade XD no soro de portadores de câncer indica uma baixa na concentração de enzima no soro sangüíneo.
Descargas
Citas
DELLA CORTE E, STIRPE F. The regulation of rat liver xanthine oxidase. Involvement of thiol groups the conversion of the enzyme activity from dehydrogenase (type D) into oxidase (type O) and purification of the enzyme. Biochem J 1972; 126: 739-745. DOI: https://doi.org/10.1042/bj1260739
STIRPE F, DELLA CORTE E. The regulation of rat liver xanthine oxidase. conversion in vitro of the enzyme activity from dehydrogenase (type O) to oxidase (type O). J Biol Chem 1969: 244: 3855-3863. DOI: https://doi.org/10.1016/S0021-9258(17)36428-1
GHEZZI P, BIANCHI M, GIANERO L, LANDOLFO S, SALMONA M. Role of reactive oxygen intermediates in the interferon-mediated depression of hepatic drug metabolism and protective etfects of N-acetylcysteine in mice. Cancer Res 1985; 45: 3444-3447.
OEI HH, ZOGANAS HC, MCCORD JM, SCHAFFER SW. Role of acetaldehyde and xanthine oxidase in ethanol-induced oxidative stress. Res Commun Chem Pathol Pharmacol 1986; 51: 195-203.
DE LAMIRANDE G, ALLARDE C. Purine-metabolizing enzymes in normal and neoplastic rat liver. Studies on 5-nucleotidase and adenosine deaminase. Proc Am Assoc Can Res 1957; 2: 224.
REI D E, LEWIN I. Adenosine deaminase, nucleoside phosphorilase and xanthine oxidase in liver tumours. British J Can 1957; 11: 494-498. DOI: https://doi.org/10.1038/bjc.1957.60
WESTERFELD WW, RICHERT DA, HILFINGER MF. Studies on xanthine oxidase during carciriogenesis by p-dimethylamirioazo-benzene. Cancer Res 1950; 10: 486-484.
BASS AD, TEPPERMAN J, RICHERT DA, WESTERFELD WW. Excretion of uric acid and aliantoiri by rats depleted of liver xanthine oxidase. Proc Soc Exptl Biol Med 1950; 73: 687-689. DOI: https://doi.org/10.3181/00379727-73-17788
PRAJDA N, MORRIS HP, WEBER G. Imbalance of purine metabolism in hepatomas of different growth rate as expressed in behavior of xanthine oxidase. Cancer Res 1976; 36: 4639-4646.
AFFONSO OR, SOUZA ASR, MITIDIERI E. Ação de sais de cobre sobre a xarltina desidrogenase na carcinogênese hepática de ratas. Rev Bras Cancerol 1984; 30: 7-11. DOI: https://doi.org/10.32635/2176-9745.RBC.1984v30n4.3465
AFFONSO OR, SOUZA ASR, MITIDIERI E. Effect of copper on blood serum xanthine dehydrogenase in rats given D-L-ethionine. Arch Geschwulstforschung 1985; 55: 167-170.
REINERS JJ, PENCE BC, BARCUS MCS, CANTU AR. 12-o-Tetradecanoylphorbol- 13-acetate-dependent induction of xanthine dehydrogenase and conversion to xanthine oxidase in murine
epidermis. Cancer Res 1987; 47: 1775-1779.
AFFONSO OR, SOUZA ASR, ASCH KH, MITIDIERI E. Atividade de xantina oxidase na carcinogênese induzida pela 1-2-dimetilidrazina. Rev Bras Cancerol 1987; 33: 29-31. DOI: https://doi.org/10.32635/2176-9745.RBC.1987v33n1.3186
MITIDIERI E, SOUZA ASR, AFFONSO OR. Xanthine oxidase activity in intestinal tumors induced by 1-2-dimethylhydrazine dihydrochloride. Arch Geschwulstforschung 1988; 58: 79-80.
AFFONSO OR, AVRES DE MOURA CV, CAVALLLARI V, MITIDIERI E. Serum and liver xanthine oxidase activitiy in tumor bearin grats and mice. An Acad Bras Cienc 1981; 53: 617-620.
MITIDIERI E, AFFONSO OR, SCHETTINO CM, RIBEIRO LP. A study of the effects of dietary Iipids upon xanthine dehydrogenase activity. An Acad Brasil Cienc 1970; 42: 633-636.
MITIDIERI E, AFFONSO OR, RIBEIRO LP. Xanthine dehydrogenase activity in starved-refed rats treated with 8-azaguanine. Acta Biol Med Germ 1974; 32: 695-699.
FRIED R. Colorimetricdetermination of xanthine dehydrogenase by tetrazolium reduction. Anal Biochem 1966; 16: 427-432. DOI: https://doi.org/10.1016/0003-2697(66)90224-7
NACHLAS MM, MARGULIES SI, SELIGMAN AM. A colorimetric method for the estimation of succinio dehydrogenase activity. J Biol Chem 1960; 235: 499-503. DOI: https://doi.org/10.1016/S0021-9258(18)69554-7
MITIDIERI E, AFFONSO OR, RIBEIRO LP. Studies on the phosphate requirement for xanthine dehydrogenase activity. 1: Phosphorus conterit and activity of different enzyme preparations. Rev Bras Biol 1969; 29: 391-397.
MITIDIERI E, AFFONSO OR, RIBEIRO LP. Role of phospholipids in xanthine dehydrogenase activity. Enzymologia 1970; 38: 161-167.
VILLELA GG, MITIDIERI E, AFFONSO OR. IritraceIlular distributior, of xanthine oxidase in rat liver. Nature 1955; 175: 1087. DOI: https://doi.org/10.1038/1751087a0
AFFONSO OR, LEMOS MF, SIMAS SM, MITIDIERI E. Characterization of the blood serum xanthine dehydrogenase by polyacrilamide-gel electrophoresis. An Acad Bras Cienc 1975; 47: 517-520.
NARAVAN KA, NARAYAN S, KUMMEROW FA. A modified disc electrophoretic method for animal blood serum proteins. J Chromatography 1964; 16: 187-193. DOI: https://doi.org/10.1016/S0021-9673(01)82454-7
LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193: 265-275. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6
LINEWEAVER H, BURK D. The determination of enzyme dissociation coristants. J Am Chem Soc 1934; 56: 658-666. DOI: https://doi.org/10.1021/ja01318a036
KIELLY KR. Purification of liver xanthine oxidase. J Biol Chem 1955; 216: 405-412. DOI: https://doi.org/10.1016/S0021-9258(19)52317-1
REMY CN, RICHERT DA, DOISY RJ, WELLS IC, WESTERFELD WW. Purification and characterization of chicken liver xanthine dehydrogenase. J Biol Chem 1955; 217: 293-305. DOI: https://doi.org/10.1016/S0021-9258(19)57181-2
BRUMBY PE, MILLER RW, MASSEY V. The content and possible catalytic significance of labile sulfide in some metalloflavoproteins. J Biol Chem 1965; 240: 2222-2228. DOI: https://doi.org/10.1016/S0021-9258(18)97450-8
RICHERT DA, WESTERFELD WW. The relationship of iron to xanthine oxidase. J Biol Chem 1954; 209: 179-189. DOI: https://doi.org/10.1016/S0021-9258(18)65544-9
HART LI, MCGARTOLL MA, CHAPMAN HR, BRAY RC. The compositiori of milk xanthine oxidase. Biochem J 1970; 116: 851-864. DOI: https://doi.org/10.1042/bj1160851
VILLELA GG, AFFONSO OR, MITIDIERI E. Xanthine dehydrogenase in bovine blood serum. Experientia 1956; 12: 477. DOI: https://doi.org/10.1007/BF02162531
VILLELA GG, AFFONSO OR, MITIDIERI E. Études sur la xanthine déhydrase du sang des bovidés. BuIl Soc Chem Biol 1957: 39: 553-559.
AFFONSO OR, MITIDIERI E, RIBEIRO LP, VILLELA GG. Blood serum xanthine oxidase of rats poisoned with carbon tetrachloride. Proc Soc Exptl Biol Med 1955; 90: 527-529. DOI: https://doi.org/10.3181/00379727-90-22087
MITIDIERI E, AFFONSO OR, RIBEIRO LP. Inhibition of xanthine dehydrogenase by semicarbazide. Biochem Pharm 1962; 11: 669-670. DOI: https://doi.org/10.1016/0006-2952(62)90127-2
MITIDIERI E, AFFONSO OR, RIBEIRO LP. In vivo inhibition of rat blood serum xanthine dehydrogenase by semicarbazide. Proc Soc Exptl Biol Med 1963; 112: 549-552. DOI: https://doi.org/10.3181/00379727-112-28102
VILLELA GG, AFFONSO OR, MITIDIERI E. lnhibitory effect of barbiturate on brairi and serum xanthine oxidase. Biochem Pharmacol 1966; 15: 1894-1896. DOI: https://doi.org/10.1016/0006-2952(66)90101-8
MITIDIERI E, AFFONSO OR. Ethanol induced inhibition in liver and blood serum xanthine dehydrogenase. Acta Biol Med Germ 1973; 31: 755-757.
MITIDIERI E, AFFONSO OR, RIBEIRO LP. Xanthine dehydrogenase activity in starved-refered rats treated with 8-azaguanine. Acta Biol Med Germ 1974; 32: 695-699.
AFFONSO OR, LEMOS MF, MITIDIERI E. Effect of nicotirtamide on the serum xanthine dehydrogenase activity during fasting. Acta Biol Med Germ 1975; 34: 1697-1699.
AFFONSO OR, LEMOS MF, SIMAS SM, MITIDIERI E. Effect of large intraperitoneal doses of fatty acids on the rat blood sérum xanthine dehydrogenase activity. Acta Biol Med Germ 1977; 36: 1191-1192. DOI: https://doi.org/10.1515/9783112650141-026
GRANGER DH, RUTILI G, MC CORD JM. Superoxide radicaIs in feIine intestinal ischemia. Gastroenterology 1981; 81: 22-29. DOI: https://doi.org/10.1016/0016-5085(81)90648-X
OEI WW, STROO WE, BURTON KP, SCHAFFER SW. A possible role of xanthine oxidase in producing oxidative stress in the heart of chronically ethanol treated rats. Res Commun Chem Pathol Pharmacol 1982; 38: 453-461.
MITIDIERI E, AFFONSO OR, SOUZA ASR. Behaviour of serum xanthine dehydrogenase in the sarcoma induced by 20-methylcholanthrene. Med Sci Res 1988; 16: 1165-1166.
