Analysis of Immunophenotypic Criteria by Flow Cytometry to Define B-Cell Chronic Lymphoproliferative Diseases

Authors

  • Lacy Cardoso de Brito Junior Universidade Federal do Pará (UFPA), Instituto de Ciências Biológicas, Laboratório de Patologia Geral – Imunopatologia e Citologia. Belém (PA), Brasil. https://orcid.org/0000-0001-9102-5817
  • Maria Beatriz da Silva Fonseca Pesquisador Autônomo. Belém (PA), Brasil. https://orcid.org/0000-0001-7307-1721
  • Ana Paula Silveira Paixão Laboratório de Patologia Clínica Dr. Paulo C. Azevedo. Belém (PA), Brasil. https://orcid.org/0000-0003-3827-507X
  • Nilmara Suellen Lopes Castro Mendes Laboratório de Patologia Clínica Dr. Paulo C. Azevedo. Belém (PA), Brasil. https://orcid.org/0000-0001-6170-8289
  • Jessica Sabrina Cordeiro Parente Fundação Centro de Hemoterapia e Hematologia do Pará (Hemopa). Belém (PA), Brasil. https://orcid.org/0000-0002-4706-4103
  • Matheus Holanda Nascimento UFPA. Laboratório de Patologia Clínica Dr. Paulo C. Azevedo. Belém (PA), Brasil. https://orcid.org/0000-0001-9351-2918

DOI:

https://doi.org/10.32635/2176-9745.RBC.2023v69n1.2734

Keywords:

lymphoproliferative disorders/diagnosis, immunophenotyping, flow cytometry

Abstract

Introduction: Flow cytometry is an important methodology for the diagnosis of chronic B-cell lymphoproliferative diseases (CBCLPD), however, sometimes the cytometrist does not find sufficient elements for the exact definition of the pathological entity involved. Objective: To analyze the reports issued to patients with chronic lymphoproliferative diseases (CLPD) tested at a private laboratory in Belém-PA, according to the classification criteria established by the studies by Matutes et al. and Craig and Foon. Method: Retrospective study with reports of patients who underwent immunophenotyping by flow cytometry for the diagnosis of CBCLPD from September 2015 to December 2019. Results: After applying the criteria by Matutes et al. and Craig and Foon to the reports analyzed, agreement was reached for 45.24% of the cases of chronic b-cell lymphocytic leukemia/small B-cell lymphocytic lymphoma, 14.29% of the cases of follicular lymphoma, 4.76% of the cases of hairy cell leukemia and 21.43% of the cases defined as “other CBCLPDs not classifiable by flow cytometry”. However, Hotelling’s hypothesis test (p=0.0409) showed a statistical difference for the definition of CBCLPD according to the criteria adopted. Conclusion: The results emphasize that even though flow cytometry is important for the characterization of CBCLPD, sometimes the cytometrist needs to include the category “other chronic B-cell lymphoproliferative diseases not classified by flow cytometry” in the report to induce the prescriber to request additional complementary exams.

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Published

2023-01-16

How to Cite

1.
Brito Junior LC de, Fonseca MB da S, Paixão APS, Mendes NSLC, Parente JSC, Nascimento MH. Analysis of Immunophenotypic Criteria by Flow Cytometry to Define B-Cell Chronic Lymphoproliferative Diseases. Rev. Bras. Cancerol. [Internet]. 2023 Jan. 16 [cited 2024 Jul. 22];69(1):e-072734. Available from: https://rbc.inca.gov.br/index.php/revista/article/view/2734

Issue

Vol. 69 No. 1 (2023): Jan./Feb./Mar.

Section

ORIGINAL ARTICLE

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