A classificação de Bethesda: uma análise morfométrica das biópsias do colo uterino

Authors

  • Carlos Roberto de Resende Miranda Departamento de Ginecologia e Obstetrícia. Universidade de Brasília (UNB), Brasília (DF), Brasil
  • Liane Deligdisch Department of Pathology and Department of Obstetrics and Gynecology, Director of Gynecology Pathology Division, Mount Sinai School of Medicine, New York - NY.
  • Joan Gil Department of Pathology, Mount Sinai School of Medicine, New York - NY.
  • Peter Remson Dottino Department of Obstetrics, Gynecology and Reproductive Science, Mount Sinai School of Medicine, New York - NY.
  • Carmel Jonathan Cohen Department of Obstetrics, Gynecology and Reproductive Science, Mount Sinai School of Medicine, New York - NY.

DOI:

https://doi.org/10.32635/2176-9745.RBC.1995v41n4.2954

Keywords:

Papillomavirus Infections, Squamous Intraepithelial Lesions, Uterine Cervical Dysplasia

Abstract

Because of the possibility of extending the Bethesda System to histologic reports, we consider in this morphometric study some of main issues involved in the HPV-related lesions, in particular the possibility of establishing a cut of between low and high-grade lesions. We compared histological and cytological features of cervical precancerous conditions by morphometric analysis. Histologic sections from 62 colposcopically guided biopsies of the cervix were studied by computerized morphometry. The cases had been grouped according to the subjective dia gnosis into three groups: normal (33 cases), Squamous Intraepithelial Lesions - Low Grade (SIL-LG, 21 cases), and Squamous Intra epithelial Lesions - High Grade (SIL-HG, 8 cases). We carried out our study using programs called DRAW and COUNT3, that considers the following parameters: area, length, maximum chord, roundness, of the nuclei of cells in the lesion, and celullarity, from the outer Iayer of cervical epithelium (superficial). After measuremens of the cells of interest, we analyzed the results by multivariate analysis and computed posterior probabilities to confirm the homogeneity of the cases in the dia gnostic groups. Area and celiularity seem to be the most reliable parameters. The posterior probability of all but six cases were higher than 0.975. Morphometric procedures fuliy support the distinction among normal squamous tissue, SIL-LG and SIL-HG.

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References

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Published

2022-10-04

How to Cite

1.
Miranda CR de R, Deligdisch L, Gil J, Dottino PR, Cohen CJ. A classificação de Bethesda: uma análise morfométrica das biópsias do colo uterino. Rev. Bras. Cancerol. [Internet]. 2022 Oct. 4 [cited 2024 Nov. 24];41(4):251-6. Available from: https://rbc.inca.gov.br/index.php/revista/article/view/2954

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ORIGINAL ARTICLE

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