Configuration of the surfaces of epithelial cells infected by human papillomavirus
DOI:
https://doi.org/10.32635/2176-9745.RBC.1999v45n1.2765Palabras clave:
Human Papillomavirus, Cervical Intraepithelicd Neoplasia, Scanning Electron Microscopy, Hybridization in SituResumen
Human papillomavirus (HPV) types were investigated hy iri situ hybridization (ISH) in biopsiesfroin J8patients with diverse pathologies ofcervix uteri (7 condylomas, 1 CINI +HPV, 3 CIN II, 4 CIN II + HPV and 3 CIN III). Cervical sniears ofthe same patients were processed by Scanning Electron Mycroscopy (SEM) in order to study the configuration of sitrfaces ofcells infected hy different HPV types. Seven condylomas, I CIN I + HPV, 3 CIN II and 3 CIN II + HPV showed alinost identical surface configurations to those of normal cells (short, thin, homogeneous microvi lli o r typicai microridgesj. This group presented low, moderate and high risk HPV types in a episomal form, while I CINII + HPV and3 CINIII with integratedHPV 16/]8 exfoliatedcells covered with gross microvilli, with variahle length and uneven distribution, oran amorphous surface. The presence of HPV 16/18 cannot be sufficient for the development of an anormal configuration, hut the integrated State ofthis viral type coiild he related to the morphological changes in cellular surface morphology. The results of this paper underline the importance of viral integration in the genesis of cervical carcinoma.
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Reid. R.; Crum, C.P.; Heschman, B.R.; Fu, Y.S.; Braun, L.; Shah, K.V.; Agronow, S.J.; Stanhope, C.R. - Genital warts and cervical câncer III. Subclinical papillomaviral infection and cervical neoplasia are linked by a spectrum of continuous morphologic and biologic changes. Cancer, 53: 943-953, 1984. DOI: https://doi.org/10.1002/1097-0142(19840215)53:4<943::AID-CNCR2820530421>3.0.CO;2-X
Stern, E.; Leely, P.M. - Carcinoma and dysplasia of the cervix: a comparison of rates for new and returning populations. Acta Cytol, 7: 357-361, 1963
Richart, R.M.; Barrow, B.A. – A follow-up study of patients with cervical dysplasia. AmJ Obstet Gynecol, 105: 386-393, 1969. DOI: https://doi.org/10.1016/0002-9378(69)90268-3
Nasiell, K.; Nassiel, M.; Vaclavinkova,A. – Behavior of moderate cervical dysplasia during long-term follow-up. Obstet Gynecol, 61: 609-614, 1983.
Stanbridge, E.J.; Der, ChJ.; Doersen, C.-J.; Nishimi, R.Y.; Peehl, D.M.; Weissman, B.E.; Wilkinson, J.E. - Human cell hybrids: analysis of transformation and tumorigenicity. Science, 215: 252-259, 1982. DOI: https://doi.org/10.1126/science.7053574
Zur Hausen, H. - Papillomavirus in human cancer. Cancer, 59: 1692-1696, 1987, DOI: https://doi.org/10.1002/1097-0142(19870515)59:10<1692::AID-CNCR2820591003>3.0.CO;2-F
Tosi,P.; Pallini,V.,Cintorino,M.; Bugnoli,M.; Covacci, A.; Petracca, R.; Syrjanen, S.; Del Vecchio, M.T.; Mantyjarvi, R.; Ruggiero, P.; Armellini, D.; Chang, F.; Syrjanen, K. - Use of antibodies against a synthetic peptide of the E6 protein of human papillomavirus (HPV) type 16 for the diagnosis of genital HPV lesions. Cytopathology, 4: 3-15, 1993. DOI: https://doi.org/10.1111/j.1365-2303.1993.tb00068.x
Walker,P.G.; Singer, A.; Dyson,J.L.; Shah, K. V.; To, A.; Coleman, D. V. - The prevalence of human papillomavirus antigen in patients with cervical intraepithelial neoplasia. Br J Câncer, 48: 99-101, 1983. DOI: https://doi.org/10.1038/bjc.1983.163
Yoshikawa, H. - Virological studies on generation of cervical carcinomas. Acta Obstet GynaecolJpn, 42: 834-843, 1990.
Popescu, N.C.; Dipaola, J.A. - Integration of human papillomavirus 16 DNA and genomic rearrangements in immortalised human keratinocyte lines. Câncer Res, 50: 1316-1323, 1990.
Yee,C.; Krishman-Hewlatt, Y.;Baker, C.C.; Schlegel, R.; Howley, P.M. - Prevalence and Expression. Of human papillomavirus sequences in human cervical carcinonias cell Unes. Am J Pathol, 1 19; 361-366. 1985.
Cullen, A.P.; Reid, R.; Campion, M.; Lorincz, A.T. Analysis of the physical State of diferente HPV-DNAs in intraepithelia! Andinvasive cervical neoplasms. J Virol, 65:606-612,1990. DOI: https://doi.org/10.1128/jvi.65.2.606-612.1991
Smotkin, D.; Prokoph,H.; Wettstein,F.O. Oncogenic and non-oncogenic human genital papillomaviruses generate theE7 niRNA by different mechanisms. J Virol, 63: 1441-1447,1989. DOI: https://doi.org/10.1128/jvi.63.3.1441-1447.1989
Pater, M.M.; Pater, A. - Human papilloma-virustype 16 and 18 sequencesin carcinoma cell lines of the cervix. Virology, 145:313-318,1985. DOI: https://doi.org/10.1016/0042-6822(85)90164-3
Wernes, B.A.; Levine, A.J.; Howley, P.M. Association of human papillomavirus type 16 and 18 E6 proteins with p53. Science, 248;76-79,1990. DOI: https://doi.org/10.1126/science.2157286
Munger, K.; Wernes,B.A.; Dyson,N. - Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product. EMBO J, 8; 4099-4105,1989. DOI: https://doi.org/10.1002/j.1460-2075.1989.tb08594.x
Levine, A. J.; Momand, J, - Tumor suppressor genes: The p53 and retinoblastoma sensitivity genes and gene products. Biochem Biophys Acta, 1032:1119-136,1990. DOI: https://doi.org/10.1016/0304-419X(90)90015-S
zurHausen, H. - Disrupted dichotomous intracellularcontrol of human papillomavirus infection in câncer of the cervix. Lancet, 343: 955-957, 1994. DOI: https://doi.org/10.1016/S0140-6736(94)90070-1
Crum, C.P.; Nagai, N.;Levine, R.V.; Silverstein, S. - In situ hybridization analysis of HPV 16DNA sequences in early cervical neoplasia. AmJ Pathol, 123: 174-182,1986.
Sternberger, L.A.; Bardy,P.H.; Cuculis,J.J,; Meyer, H.G. - The uniabeled antibody-enzyme method of immunohistocheinistiy. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antiperoxidase) and its use in Identification of spirochetas. J Histochem Cytochem, 18: 315-324,1970. DOI: https://doi.org/10.1177/18.5.315
Baker, C.C.; Phelps, W.C.; Lindgren, V.; Braun, M.J.; Gonda, M.A.; Howley, P.M. - Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinomacell lines. J Virol ,61:962-971,1987. DOI: https://doi.org/10.1128/jvi.61.4.962-971.1987
Stoler, M.H.; Rhodes, Ch.R.; Whitbeck, A.; Wolinsky, S.M.; Chow, L.T.; Broker, T.R. - Human papillomavirus type 16 and 18 gene expression in cervical neoplasias. Human Pathol,23: 1 17-128,1992. DOI: https://doi.org/10.1016/0046-8177(92)90232-R
Vousden, K.H.; Wrede, D.; Crook, T. – Human papillomavirus oncoprote in function: releasing the brakes on cell growth control. Papillomavirus Rep, 2: 1-3,1991.
Lorincz, A.T.;Quinn, A.P.;Goldsborough, M.D.; Schmidth, B .J.; Temple, G.F. - Cloning and partial DNA sequencing of the two new human papillomavirus types associated with condyloma and low-grade cervical neoplasia.J Virol, 63:2829-2834,1989. DOI: https://doi.org/10.1128/jvi.63.6.2829-2834.1989
Lehn.H.; Villa, L.L.; Marziona, F.; Hilgarth, M.; Hillemans, H.-G.; Sauer, G. - Physical State and biological activity of human papillo mavirus.! Gen Virol, 69:187-196, 1988. DOI: https://doi.org/10.1099/0022-1317-69-1-187
Ward, P.; Coleman, D.V.; Malcolm, A.D.B. Regulatory mechanisms of the papilloma-viruses. TrendsGenet,5:97-99,1988. DOI: https://doi.org/10.1016/0168-9525(89)90037-1
Rose,F.; Durst,M.; zurHausen, H. – Selective suppression of human papillomavirus trans-cription in non-tumorigenic cells by 5-azacytidine. EMBOJ, 7: 1321-1328, 1988. DOI: https://doi.org/10.1002/j.1460-2075.1988.tb02947.x
Allen, J.M.; Murphy, J.F.; Jordan, J.A.; Williams, A.E. - The use of scanning electron microscopy in cervical cytology: results from the examination of 218 patients. Scanning Electron Microsc, II: 315-320,1976.
Murphy, J.F.; Jordan, J.A.; Allen, J.M.; Williams, A.E. - Correlation of scanning electron microscopy, colposcopy and histology in 50 patients presenting abnormal cytology. J Obstet Gynaecol Br Commonw, 81:236-241,1974. DOI: https://doi.org/10.1111/j.1471-0528.1974.tb00451.x
Kenemans, P.; Davine, J.H.M.; de Haan, R.W.; van dei Zanden, P.; Vooys, G.P.; Stalk, J.G.; Stadhouders, A.M. - Cell surface morphology in intraepithelial malignancy and its precursor lesions. Scanning Electron Microsc, III: 23-26,1981.
Hodges, G.M. - Normal and neoplastic urothelium of human bladderin vivo and in vitro, an assessment of SEM studies. Scanning Electron Microsc, II: 983-990,1978.
Koss, L.G.; Domagala, W. – Configurations of surfaces of human cancercells in efusions. A review. Scanning Electron Microsc, III: 89-100,1980.
Domagala, W.; Woyke, S. – Transmission and scanning electron microscopy study of cells in effusions. Acta Cytol, 19:214-224, 1975.
Durst, M.; Kleinheiry, A.; Holtz, M.; Gissmann, L. - The physical State of human papillomavirus type 16 DNA in benign and malignant genital tumours. J Gen Virol, 66: 151-152,1985. DOI: https://doi.org/10.1099/0022-1317-66-7-1515
Durst, M.; Ozarlieva-Petrusevska, R.T.; Boukamp, P.; Fusenig, E.N.; Gissmann, L. - Molecular and cytogenetic analysis of immortalized primary keratinocytes obtained after transfection with human papillo mavirus type 16 DNA. Oncogene, 1: 251-256.1987.
Cooper, J.A.; Hunter, T. - Regulation of cell growth and transformation by tyrosine specific proteins kinasas: the search for important cellular substrate proteins. CurrentTopMicrobiolImmunol, 107: 125-161,1983. DOI: https://doi.org/10.1007/978-3-642-69075-4_4
Barton, S.E.; Hollingworth, A.; Maddox, P.H.; Edwards, R.; Cuzick,!.; McCance, D.J.; Jenkins, D.; Singer, A. - Possiblecofactors in the etiology of cervical intraepithelial neoplasia. An immunopathologic study . J Reprod Med, 34: 613-616, 1989.
Mitrani-Rosenbaun, S.; Tsviele, R.; TurKaspa, R. - Oestrogen stimulates differential transcription of human papillomavirus type 16 in SiHa cervical carcinoma cells. J Gen Virol, 70:2227-2232,1989. DOI: https://doi.org/10.1099/0022-1317-70-8-2227
Chan, M.; Popescu, N.; Woodworth, C. - Human herpesvirus 6 infects cervical epithelial cells and transactivates human papillomavirus gene expression. J Virol, 68: 1173-1178,1994. DOI: https://doi.org/10.1128/jvi.68.2.1173-1178.1994
Richart, R.M.; Masood, S.; Syrjanen, K.J.; Vassilakos, P.; Kaulman, R.H.; Meisels, A.; Olszewski, W.T.; Sakamoto, A.; Stoler,M.H.; Vooijs, G.P.; Wilbur, D.C. - Human Papillo-mavirus: lAC Task Force Summary. Acta Cytol, 42:50-58,1998. DOI: https://doi.org/10.1159/000331534
Woodworth, C.D.; Waggoner, S.; Barnes, W.; Stoler, M.H.; DiPaolo, J.A. – Human cervical and foreskin epithelial cells immortalized by human papillomavirusDNAs Exhibit dysplastic differentiation in vivo. CancerRes, 50:3709-3715, 1994.
