Receptores do Tipo Toll 2, 3 e 4 em Leucemia Linfocítica Aguda Infantil

Autores

  • Matheus Loureiro da Silva Cruz Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-1141-0254
  • Rafael Pereira dos Santos Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0003-0949-9648
  • Barbara Kunzler Souza Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-1694-1337
  • Mariane da Cunha Jaeger Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. Instituto do Câncer Infantil. Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-3465-4083
  • Camila Alves da Silva Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-4243-7767
  • Lauro José Gregianin Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0003-0788-7858
  • Jiseh Fagundes Loss Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-0088-514X
  • Rebeca Ferreira Marques Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0001-6447-1029
  • Algemir Lunardi Brunetto Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. Instituto do Câncer Infantil. Porto Alegre (RS), Brazil. https://orcid.org/0000-0003-0668-6894
  • André Tesainer Brunetto Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. Instituto do Câncer Infantil. Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-7958-1279
  • Rafael Roesler Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0001-6016-2261
  • Caroline Brunetto de Farias Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. Instituto do Câncer Infantil. Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-6435-6626

DOI:

https://doi.org/10.32635/2176-9745.RBC.2023v69n3.3676

Palavras-chave:

leucemia-linfoma linfoblástico de células precursoras, receptores Toll-like

Resumo

Introdução: A leucemia linfoblástica aguda (LLA) é o tipo de câncer mais comum em crianças e representa 80% das leucemias pediátricas. Novos alvos são necessários para melhorar as taxas de sobrevivência para doença refratária e recidivante. Há evidências acumuladas de que a sinalização de receptores Toll-Like (TLR) pode estar associada a resultados em câncer, embora pouco tenha sido descrito em leucemias. Objetivo: Analisar a expressão e a contribuição dos TLR para o desenvolvimento da LLA infantil. Método: Avaliar o efeito de agonistas específicos de TLR2, TLR3 e TLR4 na viabilidade e proliferação de linhagens celulares de LLA infantil e analisar a expressão do RNAm desses tipos de TLR em células blásticas da medula óssea no diagnóstico (D0) e na indução (D35) em pacientes LLA pediátricos. Resultados: O tratamento com agonistas de TLR reduziu a viabilidade celular das linhagens celulares Jurkat e Sup-B15. A distribuição do ciclo celular em Jurkat foi alterada, reduzindo as células poliploides e aumentando a fase sub-G1. Houve aumento na expressão dos receptores entre D0 e D35 em amostras de pacientes. Conclusão: Observou-se que a viabilidade celular das linhagens celulares respondeu com diferentes sensibilidades aos agonistas. A poliploidia associada a malignidade tumoral foi reduzida, além de o aumento da fase sub-G1 indicar aumento da apoptose. Houve diferenças na expressão de TLR em D35 entre os grupos de risco da doença. Pacientes com alta expressão de TLR2 e baixa expressão de TLR4 no D35 demonstraram pior prognóstico.

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Publicado

2023-07-06

Como Citar

1.
Cruz ML da S, Santos RP dos, Souza BK, Jaeger M da C, Silva CA da, Gregianin LJ, Loss JF, Marques RF, Brunetto AL, Brunetto AT, Roesler R, Farias CB de. Receptores do Tipo Toll 2, 3 e 4 em Leucemia Linfocítica Aguda Infantil. Rev. Bras. Cancerol. [Internet]. 6º de julho de 2023 [citado 4º de dezembro de 2024];69(3):e-053676. Disponível em: https://rbc.inca.gov.br/index.php/revista/article/view/3676

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