Toll-like Receptors 2, 3, and 4 in Childhood Acute Lymphocytic Leukemia

Authors

  • Matheus Loureiro da Silva Cruz Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-1141-0254
  • Rafael Pereira dos Santos Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0003-0949-9648
  • Barbara Kunzler Souza Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-1694-1337
  • Mariane da Cunha Jaeger Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. Instituto do Câncer Infantil. Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-3465-4083
  • Camila Alves da Silva Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-4243-7767
  • Lauro José Gregianin Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0003-0788-7858
  • Jiseh Fagundes Loss Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-0088-514X
  • Rebeca Ferreira Marques Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0001-6447-1029
  • Algemir Lunardi Brunetto Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. Instituto do Câncer Infantil. Porto Alegre (RS), Brazil. https://orcid.org/0000-0003-0668-6894
  • André Tesainer Brunetto Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. Instituto do Câncer Infantil. Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-7958-1279
  • Rafael Roesler Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. https://orcid.org/0000-0001-6016-2261
  • Dra. Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. Instituto do Câncer Infantil. Porto Alegre (RS), Brazil. https://orcid.org/0000-0002-6435-6626

DOI:

https://doi.org/10.32635/2176-9745.RBC.2023v69n3.3676

Keywords:

precursor cell lymphoblastic leukemia-lymphoma, Toll-like receptors

Abstract

Introduction: Acute lymphocytic leukemia (ALL) is the most common cancer type in children and accounts for 80% of pediatric leukemias. Novel targets are necessary to improve survival rates for refractory and relapsed disease. There is accumulating evidence that Toll-like Receptor (TLR) signaling may be associated with outcomes in cancer however little has been described in leukemias. Objective: Analyze the expression and contribution of TLRs to the development of childhood ALL. Method: To evaluate the effect of specific TLR2, TLR3, and TLR4 agonists on the viability and proliferation of childhood ALL cell lines and to analyzed the mRNA expression of these types of TLR in bone marrow blast cells at diagnosis (D0) and induction (D35) in pediatric ALL patients. Results: Treatment with TLR agonists reduced the cell viability of Jurkat and Sup-B15 cell lines. Cell cycle distribution in Jurkat was altered, reducing polyploid cells and increasing sub-G1 phase. Conclusion: It was observed that the cell viability of the cell lines responded with different sensitivities to the agonists. The polyploidy associated with tumor malignancy was reduced, in addition to the increase in the sub-G1 phase indicating an increase in apoptosis. There were differences in TLR expression at D35 between groups at risk of the disease. Patients with high expression of TLR2 and low expression of TLR4 on D35 demonstrated a worse prognosis.

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Published

2023-07-06

How to Cite

1.
Cruz ML da S, Santos RP dos, Souza BK, Jaeger M da C, Silva CA da, Gregianin LJ, Loss JF, Marques RF, Brunetto AL, Brunetto AT, Roesler R, Farias CB de. Toll-like Receptors 2, 3, and 4 in Childhood Acute Lymphocytic Leukemia. Rev. Bras. Cancerol. [Internet]. 2023 Jul. 6 [cited 2025 Apr. 19];69(3):e-053676. Available from: https://rbc.inca.gov.br/index.php/revista/article/view/3676

Issue

Vol. 69 No. 3 (2023): July/Aug./Sept.

Section

ORIGINAL ARTICLE

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